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Karyological analysis of Myosotis sylvatica agg.

Bernarda Jamnik Zupančič (2016) Karyological analysis of Myosotis sylvatica agg.. MSc thesis.

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    Previous morphological research of the Myosotis sylvatica group in the Department of Biology has shown there is another taxon present in Slovenia. The majority of the results of the measurements have placed the new taxon between the M. sylvatica and the M. decumbens. The only reliable trait between taxa is pollen size. Since pollen grain size indicates genome size we measure nuclear genome size of the representatives from the Myosotis sylvatica group from the territory of Slovenia and the neighbouring areas and count their chromosome numbers. The research includes 50 plants from Slovenia, Croatia, Austria and Italy. DNA image cytometry was used to measure nuclear genome size in the root tips. The following karyotypes were obtained: 2C = 1,21 pg for M. decumbens, 2C = 1,13 pg DNA for the new taxon and 2C = 0,62 pg DNA for M. sylvatica. Data about nuclear DNA amount was compared to the past measurements of pollen sizes. We found a positive correlation between these traits. Determining the chromosome number was less successful. Despite this, it is apparent that the new taxon has a value of chromosome number between M. sylvatica and M. decumbens.

    Item Type: Thesis (MSc thesis)
    Keywords: Wood Forget-me-not, Myosotis sylvatica agg, DNA-image cytometry, nuclear genome size, C-value, chromosome number
    Number of Pages: 52
    Language of Content: Slovenian
    Mentor / Comentors:
    Mentor / ComentorsIDFunction
    doc. dr. Simona Strgulc KrajšekMentor
    doc. dr. Jasna Dolenc KoceComentor
    Link to COBISS: http://www.cobiss.si/scripts/cobiss?command=search&base=50126&select=(ID=11362121)
    Institution: University of Ljubljana
    Department: Faculty of Education
    Item ID: 4226
    Date Deposited: 30 Nov 2016 16:16
    Last Modified: 30 Nov 2016 16:16
    URI: http://pefprints.pef.uni-lj.si/id/eprint/4226

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