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Deletion of aegerolysin gene in strain of bacteria Pseudomonas aeruginosa PA01

Melisa Tršan (2016) Deletion of aegerolysin gene in strain of bacteria Pseudomonas aeruginosa PA01. Diploma thesis.

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    Abstract

    The dissertation discusses the proteins from the aegerolysin family, specifically the RahU protein of the opportunistic pathogen bacteria, Pseudomonas aeruginosa. The biology of P. aeruginosa, which exploits its host’s weak immune system to cause numerous infections, is described. The main focus of the dissertation was the preparation of the deletion mutants. The rahU gene was deleted from the P. aeruginosa genome by replacing it with the kanymicin cassette. The gene replacement was carried out by using the Lambda Red recombinase system. The purpose of this molecular tool was to gain the rahU deletion mutants, which enable us to obtain new insights into the biology of the RahU protein.

    Item Type: Thesis (Diploma thesis)
    Keywords: aegerolysins, Pseudomonas aeruginosa, proteins RahU, opportunistic pathogen, kanamycin cassette, deletion, homologous recombination
    Number of Pages: 27
    Language of Content: Slovenian
    Mentor / Comentors:
    Mentor / ComentorsIDFunction
    doc. dr. Matej ButalaMentor
    Link to COBISS: http://www.cobiss.si/scripts/cobiss?command=search&base=50126&select=(ID=11224649)
    Institution: University of Ljubljana
    Department: Faculty of Education
    Item ID: 3905
    Date Deposited: 07 Oct 2016 14:16
    Last Modified: 07 Oct 2016 14:16
    URI: http://pefprints.pef.uni-lj.si/id/eprint/3905

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