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Speciation within the species complex of the Grayling (Hipparchia semele) on the Balkan peninsula

Anja Kristl (2014) Speciation within the species complex of the Grayling (Hipparchia semele) on the Balkan peninsula. Diploma thesis.

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    Abstract

    In this thesis we wanted to determine whether the butterfly species complex within the genus Hipparchia differ on the basis of genetic markers and the shape of their genital apparatus. By using the phylogenetic analysis, we checked the genetic relatedness between individuals based on mitochondrial COI gene and the nuclear gene GADPH. Species of the genus Hipparchia are morphologically very similar they differ only in the anatomy of the male genitalia. We cut the last part of the abdomen of the sampled males and prepared permanent preparations according to the protocol. Under the microscope, we measured the length of selected genital parts and analysed them. Mitochondrial COI gene was suitable for separating H. senthes from other species, but not of the H. semele from H. volgensis. The nuclear gene GAPDH is unsuitable for identification the studied species. Male genitalia analysis proved to be appropriate for determining the butterflies of the genus Hipparchia. Genitalia of the H. semele and H. volgensis are generally similar they differ only in the length and width of the valve. H. senthes has significantly smaller genitalia compared to the other two studied species. It is probable that incompatibility based on the size of genitalia is a major factor in the development of mechanisms of reproductive isolation among species in the H. semele complex.

    Item Type: Thesis (Diploma thesis)
    Keywords: butterflies, Lepidoptera, Hipparchia Semele, Hipparchia volgensis, Hipparchia senthes, morphology of the male genitalia, cryptic species
    Number of Pages: 50
    Language of Content: Slovenian
    Mentor / Comentors:
    Mentor / ComentorsIDFunction
    asist. dr. Rudi VerovnikMentor
    Link to COBISS: http://www.cobiss.si/scripts/cobiss?command=search&base=50126&select=(ID=10307145)
    Institution: University of Ljubljana
    Department: Faculty of Education, Biotechnical Faculty, Faculty of chemistry and chemical technology, Faculty of Natural Sciences and Engineering
    Item ID: 2617
    Date Deposited: 20 Nov 2014 13:54
    Last Modified: 20 Nov 2014 13:54
    URI: http://pefprints.pef.uni-lj.si/id/eprint/2617

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