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Hyperaccumulation of cadmium in different populations of pennycress (Thlaspi praecox) and its significance for phytoremediation

Nina Kavčič (2012) Hyperaccumulation of cadmium in different populations of pennycress (Thlaspi praecox) and its significance for phytoremediation. Diploma thesis.

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    Abstract

    The aim of our study was to compare three different populations of pennycress Thlaspi praecox (populations from Lozice, Lokovec and Mežica) when exposed to different concentrations of cadmium (Cd) in the substrate. Thlaspi praecox is indigenous to Slovenia and it has been recently discovered to hyperaccumulate Cd. In the first part of our experiments we have grown three different populations of Thlaspi praecox under greenhouse conditions. These populations were grown in substrates with different levels of Cd (0, 50, 100, 250 mg/kg Cd). We have collected the plants from substrate before the flowering period. We separated the substrate from the roots and divided the pants to shoots and roots. Plant material was washed thoroughly and freeze dried. The roots and shoots were mineralized with mixture of HNO3 and HClO4 (10:1) and we extracted the Cd from the substrate with the ammonium acetate (NH4Ac). The concentration of Cd in roots, shoots and substrate was measured by atomic absorption spectrometry (AAS). In the second part of our experiments we have collected Thlaspi praecox plant in their natural habitats (Lozice, Lokovec, Mežica) in the time of flowering and seeding. We separated the substrate from the roots and divided the pants to shoots and roots. The measurements of concentration of Cd in roots, shoots and substrate were performed by X-ray fluorescence spectrometry (XRF). The results showed that there is a difference in concentration of Cd in roots, shoots and substrates among populations collected in the natural habitats. The population from Mežica has the highest concentration of Cd in all the samples and is statistically different from Lozice and Lokovec populations. The populations from Lokovec and Ložice have TF higher than 1 while TF of Mežica population is below 1. The BAF of shoots is the highest in Mežica and Lokovec populations while BAF of roots is the highest only in Mežica population. The results from natural habitats showed that population from Lozice exceed the warning value of Cd concentration while populations from Mežica and Lokovec exceed the critical value of Cd concentration in soil. Populations from Lokovec and Mežica in their natural habitats exceed the limit which represents the Cd hyperaccumulation capability (>100 mg Cd/kg v DW). The results of our laboratory population show that the populations do not differ statistically in their biomass. By increasing Cd concentration in the substrate the biomass of roots declined in all populations. The population from Mežica has the highest Cd concentration in roots and shoots, the highest BAF and the highest total content of Cd in roots and shoots. The Mežica population has also the best phytoextraction potential however its TF is smaller than 1, which is uncommon for this population. The results showed that all populations have abilitiy to hyperaccumulate Cd because Cd concentrations in roots and shoots exceeded100 mg/kg of Cd, when Cd was added to the substrate. All populations also tolerated the highest concentration of Cd in the substrate. Based on these results we can confirm that the population of Thlaspi praecox from Mežica is best suited for phytoextraction from Cd contaminated soil.

    Item Type: Thesis (Diploma thesis)
    Keywords: Cd concentration, Cd hyperaccumulation, Thlaspi praecox, Cd phytoextraction, Lozice, Lokovec, Mežica, variability between populations
    Number of Pages: 72
    Language of Content: Slovenian
    Mentor / Comentors:
    Mentor / ComentorsIDFunction
    prof. dr. Marjana RegvarMentor
    Link to COBISS: http://www.cobiss.si/scripts/cobiss?command=search&base=50126&select=(ID=9600073)
    Institution: University of Ljubljana
    Department: Faculty of Education, Biotechnical faculty
    Item ID: 1407
    Date Deposited: 15 Apr 2013 13:05
    Last Modified: 15 Apr 2013 13:05
    URI: http://pefprints.pef.uni-lj.si/id/eprint/1407

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