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Determination of Fallopia sect. Reynoutria taxa with molecular methods

Mersiha Bjelić (2012) Determination of Fallopia sect. Reynoutria taxa with molecular methods. Diploma thesis.

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    Abstract

    In our research invasive alien knotweed taxa sampled in Slovenia were identified using selected molecular methods. The aim was to find out whether Slovene samples of Fallopia sect. Reynoutria or sect. Sermentosae taxa can be unambiguously distinguished on the basis of the combination of chosen chloroplast and nuclear DNA markers. We used PCR RFLP analysis of the trnK intron of chloroplast DNA in combination with amplification of nuclear, simple sequence repeat (SSR) or microsatellite loci, KW6, potentially diagnostic F. sachalinensis-specific marker. According to the predominantly maternal inheritance of cpDNA in crosses of Fallopia species and based on PCR RFLP profiles of the trnK intron of cpDNA we could also make assumptions about the direction of hybridization between Japanese and giant knotweed. We tried to find out if the situation on this phenomenon in Slovenia was the same as in Europe, where hybridization between these two invasive alien knotweed species is unidirectional, with F. japonica (var. japonica), in European flora present as cytologically and genetically unified female clone, as the female (maternal) parent.

    Item Type: Thesis (Diploma thesis)
    Keywords: invasive alien species, knotweed, hybrid, molecular methods, PCR, molecular markers, trnK, KW6
    Number of Pages: 70
    Language of Content: Slovenian
    Mentor / Comentors:
    Mentor / ComentorsIDFunction
    doc. dr. Simona Strgulc KrajšekMentor
    asist. dr. Sabina AnžlovarComentor
    Link to COBISS: http://www.cobiss.si/scripts/cobiss?command=search&base=50126&select=(ID=9388617)
    Institution: University of Ljubljana
    Department: Faculty of Education, Biotechnical Faculty, Faculty of Chemistry and Chemical Technology, Faculty of Natural Sciences and Engineering
    Item ID: 1085
    Date Deposited: 26 Sep 2012 11:59
    Last Modified: 04 Nov 2013 13:24
    URI: http://pefprints.pef.uni-lj.si/id/eprint/1085

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